Journal: Iranian Journal of Microbiology

Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test

doi:

Figure Lengend Snippet: The OD of sera of C. trachomatis suspected patients in rOMP2 coated ELISA plate wells.

Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by Euroimmun commercial ELISA kit.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Iranian Journal of Microbiology

Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test

doi:

Figure Lengend Snippet: The sera OD for C. trachomatis suspected patients in Euroimmun commercial ELISA kit Since the P-Value = 0.88 is greater than the significance level of a = 0.05, so there is no significant relationship between the rOMP2 and Euroimmun commercial ELISA kit.

Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by Euroimmun commercial ELISA kit.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Iranian Journal of Microbiology

Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test

doi:

Figure Lengend Snippet: Results of ELISA test with rOMP2 on 192 sera samples of C. trachomatis suspected patients.

Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by Euroimmun commercial ELISA kit.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Iranian Journal of Microbiology

Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test

doi:

Figure Lengend Snippet: Results of ELISA test with Euroimmun commercial ELISA kit on 180 sera samples of C. trachomatis suspected patients.

Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by Euroimmun commercial ELISA kit.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Iranian Journal of Microbiology

Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test

doi:

Figure Lengend Snippet: Results of ELISA with Euroimmun commercial kit and C. trachomatis rOMP2.

Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by Euroimmun commercial ELISA kit.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Case–Case Analysis Using 7 Years of Travelers' Diarrhea Surveillance Data: Preventive and Travel Medicine Applications in Cusco, Peru

doi: 10.4269/ajtmh.16-0633

Figure Lengend Snippet: Pathogen counts and frequencies for 230 total samples

Article Snippet: To determine Enterobacteriaceae susceptibility given lack of breakpoints published in CSLI, our laboratory used Staphylococcus aureus for quality control, and then established a resistance cutoff of 16 mm with a susceptible strain of E. coli 25922. , – After preparing fresh specimens for parasite identification, the remaining stool was frozen and sent to Naval Medical Research Unit No. 6 (NAMRU-6) in Lima, Peru, for additional parasite testing using microscopy and commercial enzyme-linked immunosorbent assay (ELISA) kits for Entamoeba histolytica (TechLabs, Blacksburg, VA), Giardia lamblia (BioTech Trading Partners), and Cryptosporidium (BioTech Trading Partners, Encinitas, CA).

Techniques:

Journal: Antioxidants

Article Title: Adenophora Stricta Root Extract Protects Lung Injury from Exposure to Particulate Matter 2.5 in Mice

doi: 10.3390/antiox11071376

Figure Lengend Snippet: AsE decreases the production of inflammatory mediators in the lungs. ( a – d ) Protein levels of TNF-α ( a ), IL-6 ( b ), CXCL-1 ( c ), and CXCL-2 ( d ) were quantified by ELISA. ( e , f ) mRNA levels of NF-κB ( e ) and p38 ( f ) were quantified by qPCR. a p < 0.01, b p < 0.05 versus vehicle group; c p < 0.01, d p < 0.05 versus PM 2.5 group; e p < 0.01, f p < 0.05 versus PM 2.5 + DEXA group; TNF-α, tumor necrosis factor α; IL, interleukin; CXCL, C-X-C motif chemokine ligand.

Article Snippet: Levels of matrix metalloproteinase 9 (MMP-9), MMP-12, acetylcholine, substance P, tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), C-X-C motif chemokine ligand 1 (CXCL-1), and CXCL-2 were measured using commercial ELISA kits (Mybiosource; San Diego, CA, USA), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunology Research

Article Title: The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment

doi: 10.1155/2020/2140694

Figure Lengend Snippet: rPMCs release de novo synthesized mediators upon stimulation with TLR3 ligand. rPMCs were challenged with poly(I:C) at the indicated concentrations, PGN from S. aureus (10 μ g/mL), or LPS from E. coli (100 ng/mL) for 2 (a) or 12 h (b–f). CysLT (a), IFN- α (b), IFN- β (c), TNF (d), CCL3 (e), and CXCL8 (f) were measured in cell supernatants using ELISA. Results are presented as the mean ± SD of 4 independent experiments, and each experiment was done in duplicate ( n = 8). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; n.s.: not significant.

Article Snippet: Cytokine release was assessed by ELISA commercial kits for IFN- α , IFN- β , CCL3, CXCL8 (Wuhan EIAab Science Co., Ltd, Wuhan, China), or tumor necrosis factor (TNF) (Gen-Probe Inc., San Diego, CA, USA); assay sensitivities were <5.5 pg/mL, <6.1 pg/mL, <0.058 ng/mL, <7.8 pg/mL, or <15 pg/mL, respectively.

Techniques: Synthesized, Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunology Research

Article Title: The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment

doi: 10.1155/2020/2140694

Figure Lengend Snippet: TLR3 ligand-induced release of type I IFNs from rPMCs is TBK1/IKK ε -dependent. rPMCs were preincubated with medium alone (control) or BX-795 (1 μ M) for 15 min and then exposed to poly(I:C) at 10 μ g/mL for 12 h. IFN- α (a) and IFN- β (B) concentration was measured in cell supernatants using ELISA. Data are presented as the mean ± SD of 4 independent experiments, and each experiment was done in duplicate ( n = 8). ∗∗∗ p < 0.001.

Article Snippet: Cytokine release was assessed by ELISA commercial kits for IFN- α , IFN- β , CCL3, CXCL8 (Wuhan EIAab Science Co., Ltd, Wuhan, China), or tumor necrosis factor (TNF) (Gen-Probe Inc., San Diego, CA, USA); assay sensitivities were <5.5 pg/mL, <6.1 pg/mL, <0.058 ng/mL, <7.8 pg/mL, or <15 pg/mL, respectively.

Techniques: Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunology Research

Article Title: The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment

doi: 10.1155/2020/2140694

Figure Lengend Snippet: Fc ε RI-mediated release of de novo synthesized mediators upon rPMC stimulation with TLR3 ligand. rPMCs were treated with medium alone, poly(I:C) at 10 μ g/mL, anti-IgE at 5 μ g/mL, or both poly (I:C) and anti-IgE for 2 (a) or 12 h (b–f). CysLT (a), IFN- α (b), IFN- β (c), TNF (d), CCL3 (e), and CXCL8 (f) were measured in cell supernatants using ELISA. Bars for the poly(I:C) at 10 μ g/mL and medium alone demonstrate the same data set as in . Results are presented as the mean ± SD of 4 independent experiments, and each experiment was done in duplicate ( n = 8). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; n.s.: not significant.

Article Snippet: Cytokine release was assessed by ELISA commercial kits for IFN- α , IFN- β , CCL3, CXCL8 (Wuhan EIAab Science Co., Ltd, Wuhan, China), or tumor necrosis factor (TNF) (Gen-Probe Inc., San Diego, CA, USA); assay sensitivities were <5.5 pg/mL, <6.1 pg/mL, <0.058 ng/mL, <7.8 pg/mL, or <15 pg/mL, respectively.

Techniques: Synthesized, Enzyme-linked Immunosorbent Assay